promoter methylation and gene expression in human cd34+ stem cells derived erythroid lineage by microrna

Authors

mehdi azad department of medical laboratory sciences, faculty of allied medicine, qazvin university of medical sciences, qazvin, i

farshad forooghi department of immunology, qazvin university of medical sciences, qazvin, iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی قزوین (qazvin university of medical sciences)

hassan ehteram department of pathology, school of medicine, kashan university of medical sciences, kashan, iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی قزوین (qazvin university of medical sciences)

mousa vatanmakan department of hematology, faculty of allied medicine, tehran university of medical sciences, tehran, iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی کاشان (kashan university of medical sciences)

abstract

background: stem cell differentiation is a process composed of vast variety of factors which are controlled by a network of certain mechanisms. this study aims to determine the possible role of dna methylation, a potent regulator of vhl, ecad and runx3 genes during erythroid differentiation driven by mir-451. materials and methods: to determine the methylation status of promoters and the expression levels of vhl, ecad and runx3 genes, methylation specific pcr (msp) and real-time pcr were used, respectively, on both cord blood cd34+ hematopoietic stem cells and differentiated cells. to measure the expression levels of mir-451, mirna qpcr technique was used. results: our findings demonstrated a similar methylation pattern for the target genes before and after differentiation by mir-451. however, the expression levels were significantly increased after differentiation. gene expression and surface marker analysis results further confirmed the potential of mir-451 for driving erythroid differentiation from hematopoietic stem cells. discussion: our findings ruled out dna methylation effect on the regulation of vhl, ecad, and runx3 genes during mir-451 mediated erythroid differentiation. however, having cpg islands in their promoters, these three genes are candidates to be controlled by methylation which may not able to be detected by msp method. conclusion: taken together in this study we have shown a successful erythroid differentiation mediated by mir-451 which is at least in part, independent of dna methylation. further understanding of the underlying mechanisms driven by eryhtroid differentiation may lead to therapeutic measures to alter disorders of hematopoietic stem cell differentiation.

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Journal title:

iranian journal of pediatric hematology and oncology

جلد ۷، شماره ۲، صفحات ۱۰۵-۱۱۶

Keywords

[ ' d n a m e t h y l a t i o n ' , ' e r y t h r o i d d i f f e r e n t i a t i o n ' , ' g e n e e x p r e s s i o n ' , ' m i r ' , 4 5 1 , ' s t e m c e l l ' ]

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